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價(jià)格 | ¥4800.00 |
品牌 | NewEast Biosciences |
產(chǎn)地 | 中國(guó) 武漢 |
貨號(hào) | 26903 |
規(guī)格 | 30μl |
說明書 | 說明書下載 |
Active Rac1 GTP [26903] [30 µL] |
Catalog Number: 26903 |
Gene Symbol: RAC1 |
Description: Anti Active Rac1-GTP Mouse Monoclonal Antibody |
Background: Small GTPases are a super-family of cellular signaling regulators. Rac belongs to the Rho sub-family of GTPases that regulate cell motility, cell division, and gene transcription. GTP binding increases the activity of Rac, and the hydrolysis of GTP to GDP renders it inactive. GTP hydrolysis is aided by GTPase activating proteins (GAPs), while exchange of GDP for GTP is facilitated by guanine nucleotide exchange factors (GEFs). |
Immunogen: Recombinant full length protein of active Rac1 |
Applications: IP, IHC |
Recommended Dilutions: 1 µg for 1~2 mg total cellular proteins for IP; 1:100 dilution for IHC |
Concentration: 1 mg/ml |
Host: mouse |
Format: Liquid |
Clonality: Monoclonal |
Isotype: IgM |
Purity: Purified from ascites |
Storage buffer: |
Preservative: no |
Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
Species Reactivity: Anti-active Rac1 antibody recognizes active Rac1 from vertebrates. |
Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles |
Immunoprecipitation/Western blot: |
Rac activation assay. MEF cells were treated with (lane 2) or without (lane 1) PDGF. Cell lysates were incubated with an anti active Rac monoclonal antibody (Cat # 26903) (top panel). Th precipitated active Rac was immunoblotted with an anti Rac rabbit polyclonal antibody (Cat # 21003). The bottom panel shows the Western blot with anti Rac of the cell lysates used (5% of that used in the top panel). |
Immunofluorescence: |
Immunofluorescence for the active Rac using Anti Active Rac1 GTP Mouse Monoclonal Antibody [26903] shows Rac GTP immunolabeling (green) in combination with cofilin (red) on brain tissue sections. The tissue sections were fixed with -20 ºC methanol or 4% paraformaldehyde (fixation time 1hr) and stained with antibody at 1:1000 in 0.1M Phosphate buffer with 0.3% Triton X, and 4% BSA for 24h at room temperature. Secondary antibodies were anti mouse AlexaFluor488 and anti rabbit AlexaFluor594 at 1:1000. |
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發(fā)表時(shí)間:2022年
名稱/貨號(hào):Anti- Active Rac1 Mouse Monoclonal Antibody/ 26903
影響因子:Cell Death & Disease IF=9
應(yīng)用范圍:IP
種屬:Mouse
發(fā)表時(shí)間:2022年
名稱/貨號(hào):Rac Pulldown Activation Assay Kit/ 80501
影響因子: Journal of Cell Communication and Signaling IF=4.1
應(yīng)用范圍:IP-WB
種屬:Human
發(fā)表時(shí)間:2022年
名稱/貨號(hào):Anti- Active Rac1 Mouse Monoclonal Antibody/26903
影響因子:Journal of Neuroinflammation IF=9.58
應(yīng)用范圍:IP
種屬:Rat
發(fā)表時(shí)間:2022年
名稱/貨號(hào):Anti- Active Rac1 Mouse Monoclonal Antibody/ 26903
影響因子:Journal of Clinical and Translational Hepatology IF=3.6
應(yīng)用范圍:IP
種屬:Mouse
發(fā)表時(shí)間:2022年
名稱/貨號(hào):Anti- Active Rac1 Mouse Monoclonal Antibody/26903
影響因子:Cell Death Discovery IF=7.1
應(yīng)用范圍:IP
種屬:Mouse
發(fā)表時(shí)間:2022年
名稱/貨號(hào):Anti- Active Rac1 Mouse Monoclonal Antibody/ 26903
影響因子:Journal of Cell Communication and Signaling IF=4.1
應(yīng)用范圍:IP
種屬:Mouse
發(fā)表時(shí)間:2022年
名稱/貨號(hào):Anti- Active Rac1 Mouse Monoclonal Antibody/26903
影響因子:Exp Cell Res IF=3.7
應(yīng)用范圍:IP
種屬:Human
發(fā)表時(shí)間:2017年
名稱/貨號(hào):Rac Pulldown Activation Assay Kit/ 80501
影響因子:Neuropsychopharmacology IF=7.16
應(yīng)用范圍:IP
種屬:Human
發(fā)表時(shí)間:2018年
名稱/貨號(hào):Anti- Active Rac1 Mouse Monoclonal Antibody/ 26903
影響因子:Acta Neuropathologica Communications IF=7.801
應(yīng)用范圍:IHC
種屬:Mouse
發(fā)表時(shí)間:2019 年
名稱/貨號(hào):Anti- Active Rac1 Mouse Monoclonal Antibody/ 26903
影響因子:Oncogene IF=7.971
應(yīng)用范圍:IP
種屬:Human
發(fā)表時(shí)間:2017年
名稱/貨號(hào):Anti- Active Rac1 Mouse Monoclonal Antibody /26903
影響因子:Kidney International IF=8.945
應(yīng)用范圍:IF
種屬:Mouse
發(fā)表時(shí)間:2021年
名稱/貨號(hào):Anti- Active Rac1 Mouse Monoclonal Antibody/ 26903
影響因子:Angiogenesis IF=9.596
應(yīng)用范圍:IP
種屬:Human
發(fā)表時(shí)間:2020年
名稱/貨號(hào):Anti- Active Rac1 Mouse Monoclonal Antibody/ 26903
影響因子:Angiogenesis IF=9.78
應(yīng)用范圍:IP
種屬:Human Mouse
發(fā)表時(shí)間:2021 年
名稱/貨號(hào):Anti- Active Rac1 Mouse Monoclonal Antibody/26903
影響因子:Advanced Healthcare Materials IF=9.933
應(yīng)用范圍:IHC
種屬:Human
發(fā)表時(shí)間:2020年
名稱/貨號(hào):Anti- Active Rac1 Mouse Monoclonal Antibody/ 26903
影響因子:Developmental Cell IF=10.092
應(yīng)用范圍:IP
種屬: Mouse
發(fā)表時(shí)間:2017年
名稱/貨號(hào):Anti- Active Rac1 Mouse Monoclonal Antibody /26903
影響因子:Journal Of Allergy And Clinical Immunology IF=10.228
應(yīng)用范圍:IP IF
種屬:Human Mouse
發(fā)表時(shí)間:2018年
名稱/貨號(hào):Rac Pulldown Activation Assay Kit /80501
影響因子:Cardiovascular Research IF=10.878
應(yīng)用范圍:IP-WB
種屬:
發(fā)表時(shí)間:2019年
名稱/貨號(hào):Anti- Active Rac1 Mouse Monoclonal Antibody /26903
影響因子:Nature Communications IF=11.878
應(yīng)用范圍:FC
種屬:Mouse
發(fā)表時(shí)間:2019年
名稱/貨號(hào):Anti- Active Rac1 Mouse Monoclonal Antibody/ 26903
影響因子:Nature Communications IF=12.121
應(yīng)用范圍:IP
種屬:Mouse
發(fā)表時(shí)間:2020年
名稱/貨號(hào):Anti- Active Rac1 Mouse Monoclonal Antibody/ 26903
影響因子:Nature Communications IF=12.121
應(yīng)用范圍:IP
種屬:Human
發(fā)表時(shí)間:2019年
名稱/貨號(hào):Anti- Active Rac1 Mouse Monoclonal Antibody /26903
影響因子:Nature Communications IF=14.919
應(yīng)用范圍:IHC
種屬:Xenopus
發(fā)表時(shí)間:2021年
名稱/貨號(hào):Anti- Active Rac1 Mouse Monoclonal Antibody /26903
影響因子:Nature Communications IF=14.919
應(yīng)用范圍:IHC
種屬:Mouse
發(fā)表時(shí)間:2017年
名稱/貨號(hào):Anti- Active Rac1 Mouse Monoclonal Antibody /26903
影響因子:Blood IF=22.113
應(yīng)用范圍:FC
種屬:Mouse
發(fā)表時(shí)間:2017 年
名稱/貨號(hào):Anti- Active Rac1 Mouse Monoclonal Antibody /26903
影響因子:Nature Immunology IF=23.53
應(yīng)用范圍:IF
種屬:Mouse Human
發(fā)表時(shí)間:2018年
名稱/貨號(hào):Anti- Active Rac1 Mouse Monoclonal Antibody /26903
影響因子:Cancer Discovery IF=29.497
應(yīng)用范圍:IHC
種屬:Human
發(fā)表時(shí)間:2021年
名稱/貨號(hào):Anti- Active Rac1 Mouse Monoclonal Antibody
影響因子:Cell IF=41.582
應(yīng)用范圍:IHC
種屬:Mouse
技術(shù)支持:優(yōu)狐