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Anti- EYA2 Mouse Monoclonal Antibody(peptide)

價(jià)格1600.00
品牌NewEast Biosciences   
產(chǎn)地中國(guó) 武漢
貨號(hào)26052
規(guī)格100μl
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  • EYA2 [26052]
    [100 µL]

     

    Catalog Number:  26052
    Gene Symbol:  EYA2
    Description:   Anti- EYA2 Mouse Monoclonal Antibody
    Background:   EYA2 (eyes absent homolog 2) functions as a tyrosine phosphatase that specifically dephosphorylates Tyr-142 of histone H2AX. EYA2 promotes efficient DNA repair and coactivates SIX1. Together with SIX1 and DACH2, EYA2 seems to be involved in myogenesis.
    Immunogen:   A synthetic peptide near the N-terminus of human EYA2.
    Applications:   ELISA, WB, IHC, IP
    Recommended Dilutions:    
    ELISA: 1:1000-1:5000 
    WB: 1:100-1:1000 
    IHC: 1:50-1:100
    Concentration:   1 mg/ml
    Host:   mouse
    Format:   Liquid
    Clonality:   Monoclonal
    Isotype:   IgG
    Purity:   Purified from ascites
    Storage buffer:
    Preservative:  no
    Constituents:  PBS (without Mg 2+ and Ca 2+ ), pH 7.4, 150 mM NaCl, 50% glycerol
    Species Reactivity:  Anti EYA2 antibody recognizes EYA2 of vertebrates.
    Storage Conditions:  Store at -20°C. Avoid freeze / thaw cycles.
     
    Western blot:  
    Western blot analysis of recombinant full-length EYA2 protein. Purified His-tagged EYA2 protein (Cat. # 10195) was blotted with anti EYA2 monoclonal antibody (Cat. # 26052).
    Immunohistochemistry:    
    Immunohistochemical analysis of paraffin-embedded human adenocarcinoma tissue with anti EYA2 monoclonal antibody (Cat. # 26052). Tissue section was fixed with formaldehyde and blocked with 1% serum for 15 min at 37 °C. Antigen retrieval was by heat mediation in citrate buffer (pH6). Samples were then incubated with primary antibody (1:50) overnight at 4°C. A HRP-conjugated Goat anti-mouse IgG (dilution 1:50) was used as secondary antibody.

     

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