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Anti- Vimentin Mouse Monoclonal Antibody

價(jià)格1600.00
品牌NewEast Biosciences    
產(chǎn)地中國(guó) 武漢
貨號(hào)26054
規(guī)格100μl
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  • Vimentin [26054]
    [100 µL]

     

    Catalog Number:  26054
    Gene Symbol:  VIM
    Description:   Anti Vimentin Mouse Monoclonal Antibody
    Background:   Vimentin is an intermediate filament protein found in various non-epithelial cells, especially in mesenchymal cells. It is often used as a marker of mesenchymally-derived cells or cells undergoing an epithelial-to-mesenchymal transition (EMT) during both normal development and metastatic progression.
    Immunogen:   A synthetic peptide from the internal region of human vimentin protein.
    Applications:   ELISA, WB, IHC, IP
    Recommended Dilutions:   
    ELISA: 1:1000-1:5000 
    WB: 1:100-1:1000 
    IHC: 1:50-1:100
    Concentration:   1 mg/ml
    Host:   mouse
    Format:   Liquid
    Clonality:   Monoclonal
    Isotype:   IgG
    Purity:   Purified from ascites
    Storage buffer:
    Preservative:  no
    Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
    Species Reactivity:  Anti vimentin antibody recognizes vimentin of vertebrates.
    Storage Conditions:  Store at -20°C. Avoid freeze / thaw cycles
     
    Western blot:  
    Western blot analysis of recombinant vimentin protein. Purified His-tagged vimentin protein (Cat.#10190) was blotted with anti vimentin monoclonal antibody (Cat.# 26054).

     

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    Immunohistochemical analysis of  paraffin-embedded human renal cancer tissue  

    with anti-vimentin monoclonal antibody (Cat.  #26054). Tissue samples were fixed 

    with  formaldehyde and blocked with 1% serum for 15  min at 37 °C. Antigen retrieval was by heat  mediation in citrate buffer (pH6). Samples were  then incubated with primary antibody (1:100  dilution) overnight at 4°C.A HRP-conjugated  Goat anti-mouse IgG (1:50 dilution) was used as  secondary antibody

     

     

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