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Anti- PIK3CA(D549N) Mouse Monoclonal Antibody

價(jià)格¥4800.00
品牌NewEast Biosciences   
產(chǎn)地中國(guó) 武漢
貨號(hào)26215
規(guī)格100ul
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  • PIK3CA(D549N) [26215]
    [100ul]

     

    Catalog Number:  26215
    Gene Symbol:  PIK3CA, CLOVE, CWS5, MCAP, MCM, MCMTC, PI3K, p110-alpha
    Description:   Anti PIK3CA(D549N) Mouse Monoclonal Antibody
    Background:   Phosphatidyl 3-kinases (PI3K) are a family of lipid kinases involved in many cellular processes, including cell growth, proliferation, differentiation, motility, and survival. It is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. Mutants of PIK3CA has been found in the pathogenesis of several cancers, including colon cancer, gliomas, gastric cancer, breast cancer, endometrial cancer, and lung cancer.
    Immunogen:   A synthetic peptide from the internal region of PIK3CA which includes the mutation of D549N, human origin.
    Applications:   ELISA, WB, IHC
    Recommended Dilutions:   
    ELISA: 1:1000-1:5000 
    WB: 1:500-1:1000 
    IHC: 1:50-1:100
    Concentration:   0.5 mg/ml
    Host:   Mouse
    Format:   Liquid
    Clonality:   Monoclonal
    Isotype:   
    Purity:   Purified from ascites
    Storage buffer:
    Preservative:  no
    Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
    Species Reactivity:  Recognizes D549N mutant, but not wild-type PIK3CA of vertebrates.
    Storage Conditions:   Store at -20°C. Avoid freeze / thaw cycles
     
    Western blot:  
    Western blot analysis of recombinant PIK3CA(D549N) and wildtype proteins. Purified His-tagged PIK3CA(D549N) protein (lane 2) and corresponding wildtype protein (lane 1) were blotted with Anti PIK3CA(D549N) monoclonal antibody (Cat. #26215).
    Immunofluorescence:  
    Immunofluorescence of cells expressing PIK3CA proteins with Anti PIK3CA(D549N) antibody. HEK293T cells were transfected with pCDNA3-GFP PIK3CA(WT) plasmid (left column) or pCDNA3-GFP PIK3CA(D549N) plasmid (right column), then fixed and stained with Anti PIK3CA(D549N) monoclonal antibody (Cat. #26215).

     

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