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Anti- β catenin(G38D) Mouse Monoclonal Antibody

價(jià)格¥4800.00
品牌NewEast Biosciences    
產(chǎn)地中國 武漢
貨號26169
規(guī)格100ul
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  • β catenin(G38D) [26169]
    [100 µL]

    Catalog Number:  26169
    Gene Symbol:  CTNNB1, CTNNB
    Description:   Anti β catenin(G38D) Mouse Monoclonal Antibody
    Background:   Beta-catenin(or β catenin) is a protein that in humans is encoded by the CTNNB1 gene. β catenin is a subunit of the cadherin protein complex and also acts as an intracellular signal transducer in the Wnt signaling pathway. Deregulation of beta-catenin signaling is an important event in the genesis of a number of malignancies, such as colon cancer, melanoma, hepatocellular carcinoma, ovarian cancer, endometrial cancer, medulloblastoma pilomatricomas, and prostate cancer.
    Immunogen:   A synthetic peptide from the internal region of β catenin which includes the mutation of G38D, human origin.
    Applications:   ELISA, WB, IF, IHC
    Recommended Dilutions:   
    ELISA: 1:1000-1:5000 
    WB: 1:500-1:2000 
    IHC: 1:50-100
    Concentration:   1 mg/ml
    Host:   Mouse
    Format:   Liquid
    Clonality:   Monoclonal
    Isotype:   
    Purity:    Purified from ascites
    Storage buffer:
    Preservative:  no
    Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
    Species Reactivity:  Recognizes β catenin(G38D), but not wild type β catenin protein from vertebrates.
    Storage Conditions:   Store at -20°C. Avoid freeze / thaw cycles
     
    Western blot:  
    Western blot analysis of recombinant β catenin(G38D) and wildtype proteins. Purified His-tagged β catenin(G38D) protein (amino acids 1-76, lane 2) and corresponding wild type protein (lane 1) were blotted with anti β catenin(G38D) mouse monoclonal antibody (Cat. #26169).
    Immunofluorescence:   
    Immunofluorescence of cells expressing β catenin proteins with anti β catenin(G38D) antibody. HEK293T cells were transfected with pCDNA3-GFP CTNNB1(WT) plasmid (left column) or pCDNA3-GFP-CTNNB1(G38D) plasmid (right column), then fixed and stained with anti β catenin(G38D) monoclonal antibody (Cat. #26169).

     

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