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Anti- RHEB(Y35N) Mouse Monoclonal Antibody

價(jià)格¥4800.00
品牌NewEast Biosciences   
產(chǎn)地中國(guó) 武漢
貨號(hào)26338
規(guī)格100ul
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  • RHEB(Y35N) [26338]
    [100ul]

     

    Catalog Number:  26338
    Gene Symbol:  RHEB
    Description:   Anti RHEB(Y35N) Mouse Monoclonal Antibody
    Background:   RheB(Ras Homolog Enriched in Brain) is an evolutionarily conserved member of the Ras-family of small GTP-binding proteins originally found to be rapidly induced by synaptic activity in the hippocampus following seizure. While it is expressed at relatively high levels in the brain, RheB is widely expressed in other tissues and may be induced by growth factor stimulation. RheB has an important role in regulating the insulin/TOR signaling pathway. The mammalian target of rapamycin, mTOR, is a serine/threonine protein kinase that acts as a sensor for ATP and amino acids, balancing the availability of nutrients with translation and cell growth. The tuberin/hamartin (TSC2/TSC1) complex inhibits mTOR activity indirectly by inhibiting RheB via tuberin's GAP activity.
    Immunogen:   A synthetic peptide from the internal region of RHEB which includes the mutation of Y35N, human origin.
    Applications:   ELISA, WB, IHC
    Recommended Dilutions:   
    ELISA: 1:1000-1:2000 
    WB: 1:500-1:1000 
    IHC: 1:50-1:100
    Concentration:   1 mg/ml
    Host:   Mouse
    Format:   Liquid
    Clonality:   Monoclonal
    Isotype:   
    Purity:   Purified from ascites
    Storage buffer:
    Preservative:  no
    Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
    Species Reactivity:  Recognizes Y35N mutant, but not wild-type RHEB of vertebrates.
    Storage Conditions:   Store at -20°C. Avoid freeze / thaw cycles
     
    Western blot:  
    Western blot analysis of recombinant RHEB(Y35N) and wildtype proteins. Purified His-tagged RHEB(Y35N) protein (lane2) and corresponding wild-type protein (lane1) were blotted with Anti RHEB(Y35N) monoclonal antibody (Cat. #26338).
    Immunofluorescence:  
    Immunofluorescence of cells expressing RHEB proteins with Anti RHEB(Y35N) antibody. HEK293T cells were transfected with pCDNA3-GFP RHEB(WT) plasmid (left column) or pCDNA3-GFP RHEB(Y35N) plasmid (right column), then fixed and stained with Anti RHEB(Y35N) monoclonal antibody (Cat. #26338).
    Related Products
    Catalog# Name Size Price
    26910 Active RheB GTP Monoclonal Antibody  30 μL  ¥4800
    81201 RheB Activation Assay Kit  20 assays  ¥6800
    26015 Anti-RheB Mouse Monoclonal Antibody  100 μL  ¥2800
    21098 Anti-RheB Rabbit Polyclonal Antibody  100 μL  ¥2600

     

     

     


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