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Anti-BRAF(D594G) Mouse Monoclonal Antibody

價(jià)格¥4800.00
品牌NewEast Biosciences    
產(chǎn)地中國 武漢
貨號(hào)26200
規(guī)格100ul
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  • BRAF(D594G) [26200]
    [100ul]

     

    Catalog Number:  26200
    Gene Symbol:  BRAF, BRAF1, RAFB1
    Description:   Anti BRAF(D594G) Mouse Monoclonal Antibody
    Background:   BRAF belongs to a family of serine-threonine protein kinases. As a part of a signaling pathway known as the the RAS/MAPK pathway, it plays an important part in many cellular processes. The processes include cell proliferation, differentiation and transcriptional regulation. Mutations in the BRAF gene cause diseases. Inherited mutations in BRAF cause cardiofaciocutaneous syndrome. Acquired mutations in BRAF have been found in cancers.
    Immunogen:   A synthetic peptide from the internal region of BRAF which includes the mutation of D594G, human origin.
    Applications:   ELISA, WB
    Recommended Dilutions:   
    ELISA: 1:1000-1:5000 
    WB: 1:500-1:1000
    Concentration:   1 mg/ml
    Host:   Mouse
    Format:   Liquid
    Clonality:   Monoclonal
    Isotype:   
    Purity:   Purified from ascites
    Storage buffer:
    Preservative:  no
    Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
    Species Reactivity:  Recognizes D594G mutant, but not wild-type BRAF of vertebrates.
    Storage Conditions:   Store at -20°C. Avoid freeze / thaw cycles
     
    Western blot:  
    Western blot analysis of recombinant BRAF(D594G) and wildtype proteins. Purified His-tagged BRAF(D594G) protein (lane 2) and corresponding wildtype protein (lane 1) were blotted with Anti BRAF(D594G) monoclonal antibody (Cat. #26200).

     

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