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Anti- MEK1(N382H) Mouse Monoclonal Antibody

價(jià)格¥4800.00
品牌NewEast Biosciences   
產(chǎn)地中國(guó) 武漢
貨號(hào)26270
規(guī)格100ul
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  • MEK1(N382H) [26270]
    [100ul]

    Catalog Number:  26270
    Gene Symbol:  MAP2K1; MAPKK1; MEK1; MKK1; PRKMK1
    Description:   Anti MEK1(N382H) Mouse Monoclonal Antibody
    Background:   MEK1(also known as extracellular signal-regulated kinases, ERKs) plays important role in the MAP kinase cascade, which transduces multiple extracellular signals to control cell growth, proliferation and differentiation. Activated upon growth factors stimulation, MEK1 phosphorylates MAPK3/ERK1 and MAPK1/ERK2, thus activates the MAP pathway and regulates transcription. Abnormal of the MEK1 protein, including point mutations, are implicated in diseases such as cardiofaciocutaneous syndrome (CFC) and melanoma.
    Immunogen:   A synthetic peptide from the internal region of MEK1 which includes the mutation of N382H, human origin.
    Applications:   ELISA, WB, IHC
    Recommended Dilutions:   
    ELISA: 1:1000-1:5000 
    WB: 1:500-1:1000 
    IHC: 1:50-1:100
    Concentration:   1 mg/ml
    Host:   Mouse
    Format:   Liquid
    Clonality:   Monoclonal
    Isotype:   
    Purity:   Purified from ascites
    Storage buffer:
    Preservative:  no
    Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
    Species Reactivity:  Recognizes N382H mutant, but not wild-type MEK1 of vertebrates.
    Storage Conditions:   Store at -20°C. Avoid freeze / thaw cycles
     
    Western blot:  
    Western blot analysis of recombinant MEK1(N382H) and wildtype proteins. Purified His-tagged MEK1(N382H) protein (lane 2) and corresponding wildtype protein (lane 1) were blotted with Anti MEK1(N382H) monoclonal antibody (Cat. #26270).
    Immunofluorescence:  
    Immunofluorescence of cells expressing MEK1 proteins with Anti MEK1(N382H) antibody. HEK293T cells were transfected with pCDNA3-GFP MEK1(WT) plasmid (left column) or pCDNA3-GFP MEK1(N382H) plasmid (right column), then fixed and stained with Anti MEK1(N382H) monoclonal antibody (Cat. #26270).

     

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