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Anti- NOTCH1(M2011R) Mouse Monoclonal Antibody

價(jià)格¥4800.00
品牌NewEast Biosciences   
產(chǎn)地中國 武漢
貨號26444
規(guī)格100μl
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  • NOTCH1(M2011R) [26444]
    [100ul]

     

    Catalog Number:  26444
    Gene Symbol:  NOTCH1, AOS5, AOVD1,TAN1, hN1
    Description:   Anti NOTCH1(M2011R) Mouse Monoclonal Antibody
    Background:   NOTCH1 is a human gene encoding a single-pass transmembrane receptor. It is a member of the NOTCH family. NOTCH family members play a role in a variety of developmental processes by controlling cell fate decisions. Diseases associated with NOTCH1 include aortic valve disease 1 and adams-oliver syndrome 5, and so on. An important paralog of this gene is NOTCH2.
    Immunogen:   A synthetic peptide from the internal region of NOTCH1 which includes the mutation of M2011R, human origin.
    Applications:   ELISA, WB, IF, IHC
    Recommended Dilutions:   
    ELISA: 1:1000-1:2000 
    WB: 1:500-1:1000 
    IF: 1:50-1:100 
    IHC: 1:50-1:100
    Concentration:   1 mg/ml
    Host:   Mouse
    Format:   Liquid
    Clonality:   Monoclonal
    Isotype:   
    Purity:   Purified from ascites
    Storage buffer:
    Preservative:  no
    Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
    Species Reactivity:  Recognizes M2011R mutant, but not wild-type NOTCH1 of vertebrates.
    Storage Conditions:   Store at -20°C. Avoid freeze / thaw cycles
     
    Western blot:  
    Western blot analysis of recombinant NOTCH1(M2011R) and wildtype proteins. Purified His-tagged NOTCH1(M2011R) protein (lane1) and corresponding wild-type protein (lane2) were blotted with Anti NOTCH1(M2011R) monoclonal antibody (Cat. #26444).
    Immunofluorescence:  
    Immunofluorescence of cells expressing NOTCH1 proteins with Anti NOTCH1(M2011R) antibody. HEK293T cells were transfected with pCDNA3-GFP NOTCH1(WT) plasmid (left column) or pCDNA3-GFP NOTCH1(M2011R) plasmid (right column), then fixed and stained with Anti NOTCH1(M2011R) monoclonal antibody (Cat. #26444).


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