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Anti- RAS(G13V) Mouse Monoclonal Antibody

價(jià)格¥4800.00
品牌NewEastBiosciences   
產(chǎn)地中國(guó) 武漢
貨號(hào)26477
規(guī)格100μl
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  • RAS(G13V) [26477]
    [100ul]

     

    Catalog Number:  26477
    Gene Symbol:  H-Ras; K-Ras; N-Ras
    Description:   Anti RAS(G13V) Mouse Monoclonal Antibody
    Background:   Ras protein is a membrane-associated GTPase signaling protein that regulates proliferation, differentiation, and cell survival. Ras G13V mutation result in decreased GTPase activity and constitutive signaling, and is correlated with the development of pancreatic intraepithelial neoplasia and lung lesions.
    Immunogen:   A synthetic peptide from the internal region of Ras which includes the mutation of G13V, human origin.
    Applications:   ELISA, WB, IHC
    Recommended Dilutions:   
    ELISA: 1:1000-1:5000 
    WB: 1:500-1:1000 
    IHC: 1:50-1:100
    Concentration:   1 mg/ml
    Host:   Mouse
    Format:   Liquid
    Clonality:   Monoclonal
    Isotype:   
    Purity:   Purified from ascites
    Storage buffer:
    Preservative:  no
    Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
    Species Reactivity:  Recognizes G13V mutant, but not wild-type RAS of vertebrates.
    Storage Conditions:   Store at -20°C. Avoid freeze / thaw cycles
     
    Western blot:  
    Western blot analysis of recombinant Ras proteins. Purified His-tagged RAS(G13V) protein (lane 1), corresponding wildtype protein (lane 2), His-tagged Ras(G13S) protein (lane 3), His-tagged Ras(G13C) protein (lane 4), His-tagged Ras(G13R) protein (lane 5), His-tagged Ras(G13A) protein (lane 6) were blotted with Anti RAS(G13V) monoclonal antibody (Cat. #26477).
    Immunofluorescence:  
    Immunofluorescence of cells expressing Ras proteins with Anti RAS(G13V) antibody. HEK293T cells were transfected with pCDNA3-GFP-RAS(G13V) plasmid ,pCDNA3-GFP Ras(WT) plasmid, pCDNA3-GFP Ras(G13S) plasmid, pCDNA3-GFP Ras(G13C) plasmid, pCDNA3-GFP Ras(G13R) plasmid, pCDNA3-GFP Ras(G13A) plasmid or pCDNA3-GFP Ras(G13D) plasmid, then fixed and stained with Anti RAS(G13V) monoclonal antibody (Cat. #26477).

     

     

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