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Anti-EGFR (L858Q) Mouse Monoclonal Antibody

價格¥4800.00
品牌NewEast Biosciences   
產(chǎn)地中國 武漢
貨號26221
規(guī)格100ul
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    EGFR (L858Q)

    Catalog Number: 26221

    Gene Symbol: EGFR/HER1/ERBB1

    Description: Anti-EGFR (L858Q) Mouse Monoclonal Antibody

     

     

     

     


    Background: EGFR is a receptor tyrosine kinase         

    binding ligands of the EGF family and 

    activating several signaling cascades to 

    convert extracellular cues into appropriate 

    cellular responses. It activates at least 4 major 

    downstream signaling cascades including the 

    RAS-RAF-MEK-ERK, PI3 kinase-AKT, 

    PLCgamma-PKC and STATs modules. Defects 

    in EGFR are associated with lung cancer (LNCR).

    Immunogen: A synthetic peptide from the 

    internal region of EGFR which includes the 

    mutation of L858Q, human origin. 

    Tested applications: ELISA, WB, IF, IHC

    Recommended dilutions:

    ELISA:  1:1000-1:5000

    WB:     1:500-1:1000

    IHC:     1:50-1:100

    Concentration: 1 mg/ml

    Host: Mouse

    Clonality: Monoclonal

    Purity: Purified from ascites

    Format: Liquid

    Storage buffer: 

    Preservative: no

    Constituents: PBS (without Mg2+ and Ca2+), 

    pH 7.4, 150 mM NaCl, 50% glycerol

    Species Reactivity: Recognizes EGFR (L858Q), 

    but not wild type EGFR protein from vertebrates.

    Storage Conditions: Store at -20°C. Avoid 

    freeze / thaw cycles.

    Western blot:  

    360截圖20191120124055745.jpg

     

    Western blot analysis of recombinant EGFR

    (L858Q) and wildtype proteins. Purified 

    His-tagged EGFR (L858Q) protein 

    (aa702-987, lane 1) and corresponding 

    wild-type protein (aa702-987, lane 2) were 

    blotted with anti-EGFR (L858Q) mouse 

    antibody (Cat. #26221).

     

     

     

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