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Anti- IDH1(R132H) Mouse Monoclonal Antibody

價格¥4800.00
品牌NewEast Biosciences   
產(chǎn)地中國 武漢
貨號26081
規(guī)格100ul
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  • IDH1(R132H) [26081]
    [100 µL]

    Catalog Number:  26081
    Gene Symbol:  IDH1
    Description:   Anti IDH1(R132H) Mouse Monoclonal Antibody
    Background:   Isocitrate dehydrogenase (IDH) catalyzes the oxidative decarboxylation of isocitrate to 2-oxoglutarate. Mutations of Arg-132 in IDH to Cys, His, Leu or Ser abolish magnesium binding and the conversion of isocitrate to alpha-ketoglutarate. Instead, alpha-ketoglutarate is converted to R-2-hydroxyglutarate. Elevated levels of R-2-hydroxyglutarate are correlated with an elevated risk of malignant brain tumors.
    Immunogen:   Recombinant protein of human IDH1 which includes the mutation of R132H.
    Applications:   ELISA, WB, IHC, IP
    Recommended Dilutions:    
    ELISA: 1:1000-1:5000 
    WB: 1:100-1:1000 
    IHC: 1:50-1:100
    Concentration:   1 mg/ml
    Host:   mouse
    Format:   Liquid
    Clonality:   Monoclonal
    Isotype:   IgG
    Purity:   Purified from ascites
    Storage buffer:
    Preservative:  no
    Constituents:  PBS (without Mg 2+ and Ca 2+ ), pH 7.4, 150 mM NaCl, 50% glycerol
    Species Reactivity:  Anti IDH1(R132H) antibody recognizes IDH1(R132H) of vertebrates.
    Storage Conditions:  Store at -20°C. Avoid freeze / thaw cycles
     
    Western blot:  
    Western blot analysis of recombinant IDH1(R132H) and wild-type proteins. Purified His-tagged IDH1(R132H) protein (Cat.#10199) and wild-type protein (amino acid 1-240) (Cat.#10198) were blotted with anti IDH1(R132H) mouse antibody (Cat.# 26081)(Left panel) or with anti IDH1 mouse antibody (Cat. #26136 right panel).
    Immunofluorescence:    
    Immunofluorescence of cells expressing IDH1 proteins with anti-IDH1(R132H) antibody. HEK293T cells were transfected with pCDNA3-GFP IDH1(WT) plasmid (left column) or pCDNA3-GFP IDH1(R132H) plasmid (right column), then fixed and stained with Anti IDH1(R132H) monoclonal antibody (Cat. #26081).

     

    Related Products
    Catalog# Name Size Price
    26136 Anti IDH1 Mouse Monoclonal Antibody 100 μL  ¥4800
    26406 Anti IDH1(R132P) Mouse Monoclonal Antibody 100 μL ¥4,800
    26160 Anti IDH1(R132S) Mouse Monoclonal Antibody 100 μL ¥4,800
    26081 Anti IDH1(R132H) Mouse Monoclonal Antibody  100 μL  ¥4800

     

     

     


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