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Anti- IDH1 Mouse Monoclonal Antibody

價格¥1600.00
品牌NewEast Biosciences   
產(chǎn)地中國 武漢
貨號26136
規(guī)格100μl
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  • IDH1 [26136]
    [100 µL]

     

    Catalog Number:  26136
    Gene Symbol:  IDH1
    Description:   Anti IDH1 Mouse Monoclonal Antibody
    Background:   Isocitrate dehydrogenase (IDH) catalyzes the oxidative decarboxylation of isocitrate to 2-oxoglutarate. Mutations of Arg-132 in IDH to Cys, His, Leu or Ser abolish magnesium binding and the conversion of isocitrate to alpha-ketoglutarate. Instead, alpha-ketoglutarate is converted to R-2-hydroxyglutarate. Elevated levels of R-2-hydroxyglutarate are correlated with an elevated risk of malignant brain tumors.
    Immunogen:   Recombinant proteins of human IDH1 (amino acids 1-240).
    Applications:   ELISA, WB, IHC, IP
    Recommended Dilutions:   
    ELISA: 1:1000-1:5000 
    WB: 1:100-1:1000 
    IHC: 1:50-1:100
    Concentration:   1 mg/ml
    Host:   mouse
    Format:   Liquid
    Clonality:   Monoclonal
    Isotype:   IgG
    Purity:   Purified from ascites
    Storage buffer:
    Preservative:  no
    Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
    Species Reactivity:  Anti IDH1 antibody recognizes IDH1 of vertebrates.
    Storage Conditions:  Store at -20°C. Avoid freeze / thaw cycles
     
    Western blot:  
    Western blot analysis of recombinant IDH1 protein. Purified His-tagged IDH1 protein (amino acids 1-240) (Cat.#10198) was blotted with anti IDH1 mouse antibody (Cat.# 26136).

     

    Related Products
    Catalog# Name Size Price
    26136 Anti IDH1 Mouse Monoclonal Antibody 100 μL  ¥4800
    26406 Anti IDH1(R132P) Mouse Monoclonal Antibody 100 μL ¥4,800
    26160 Anti IDH1(R132S) Mouse Monoclonal Antibody 100 μL ¥4,800
    26081 Anti IDH1(R132H) Mouse Monoclonal Antibody  100 μL  ¥4800

     

    Publications:
    1.  Specific monoclonal antibodies against IDH1/2 mutations as diagnostic tools for gliomas
        Brain Tumor Pathology. January 2015, Volume 32, Issue 1, pp 3-11
    2.  Mutant IDH inhibits HNF-4α to block hepatocyte differentiation and promote biliary cancer
        Nature 513, 110–114 (04 September 2014)
    3.  Metabolomic comparison between cells overexpressing isocitrate dehydrogenase 1 and 2 mutants and the effects of an inhibitor on the metabolism
        Journal of Neurochemistry. Volume 132, Issue 2, pages 183–193, January 2015

     

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