neweastbio@126.com
PRODUCT CENTER
價(jià)格 | ¥4800.00 |
品牌 | NewEast Biosciences |
產(chǎn)地 | 中國(guó) 武漢 |
貨號(hào) | 26165 |
規(guī)格 | 100ul |
說(shuō)明書 | 說(shuō)明書下載 |
IDH2 (R140Q) [26165]
[100ul
Catalog Number: 26165 |
Gene Symbol: IDH2; D2HGA2; ICD-M; IDH; IDHM; IDP; IDPM; mNADP-IDH |
Description: Anti IDH2(R140Q) Mouse Monoclonal Antibody |
Background: Isocitrate dehydrogenase (IDH) catalyzes the oxidative decarboxylation of isocitrate to 2-oxoglutarate. Mutations of Arg-132 in IDH to Cys, His, Leu or Ser abolish magnesium binding and the conversion of isocitrate to alpha-ketoglutarate. Instead, alpha-ketoglutarate is converted to R-2-hydroxyglutarate. Elevated levels of R-2-hydroxyglutarate are correlated with an elevated risk of malignant brain tumors. |
Immunogen: A synthetic peptide from the internal region of IDH2 which includes the mutation of R140Q, human origin. |
Applications: ELISA, WB, IF, IHC |
Recommended Dilutions: ELISA: 1:1000-1:5000 WB: 1:500-1:1000 IF: 1:50-1:100 IHC: 1:50-1:100 |
Concentration: 1 mg/ml |
Host: Mouse |
Format: Liquid |
Clonality: Monoclonal |
Isotype: |
Purity: Purified from ascites |
Storage buffer: |
Preservative: no |
Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
Species Reactivity: Recognizes R140Q mutant, but not wild-type IDH2 of vertebrates. |
Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles |
Western blot: |
Western blot analysis of recombinant IDH2(R140Q) and wildtype proteins. Purified His-tagged IDH2(R140Q) protein (lane 2) and corresponding wildtype protein (lane 1) were blotted with anti IDH2(R140Q) monoclonal antibody (Cat. #26165). |
Immunofluorescence: |
Immunofluorescence of cells expressing IDH2 proteins with anti IDH2(R140Q) antibody. HEK293T cells were transfected with pCDNA3-GFP-IDH2 (WT) plasmid (left column) or pCDNA3-GFP-IDH2(R140Q) plasmid (right column), then fixed and stained with anti-IDH2(R140Q) monoclonal antibody (Cat. #26165). |
Related Products | |||
Catalog# | Name | Size | Price |
26231 | Anti IDH2(R172G) Mouse Monoclonal Antibody | 100 μL | ¥4800 |
26164 | Anti IDH2(R172W) Mouse Monoclonal Antibody | 100 μL | ¥4,800 |
26163 | Anti IDH2(R172K) Mouse Monoclonal Antibody | 100 μL | ¥4,800 |
26408 | Anti IDH2(R172S) Mouse Monoclonal Antibody | 100 μL | ¥4800 |
26165 | Anti IDH2 (R140Q) Mouse Monoclonal Antibody | 100 μL | ¥4,800 |
26231 | Anti IDH2(R172G) Mouse Monoclonal Antibody | 100 μL | ¥4,800 |
26084 | Anti IDH2(R172H) Mouse Monoclonal Antibody | 100 μL | ¥4800 |
Publications: |
1. Mutant IDH inhibits HNF-4α to block hepatocyte differentiation and promote biliary cancer Nature 513, 110–114 (04 September 2014) |
2. Metabolomic comparison between cells overexpressing isocitrate dehydrogenase 1 and 2 mutants and the effects of an inhibitor on the metabolism Journal of Neurochemistry. Volume 132, Issue 2, pages 183–193, January 2015 |
發(fā)表時(shí)間:2019-06-01
名稱/貨號(hào):Anti -IDH2 (R140Q) Mouse Monoclonal Antibody /26165
影響因子:Cell Res
應(yīng)用范圍:IHC(作為臨床診斷的應(yīng)用工具)
種屬:Human(AML)
技術(shù)支持:優(yōu)狐