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價(jià)格 | ¥4800.00 |
品牌 | NewEast Biosciences |
產(chǎn)地 | 中國(guó) 武漢 |
貨號(hào) | 26163 |
規(guī)格 | 100μl |
說(shuō)明書 | 說(shuō)明書下載 |
IDH2(R172K) [26163]
[100 µL]
Catalog Number: 26163 |
Gene Symbol: IDH2; D2HGA2; ICD-M; IDH; IDHM; IDP; IDPM; mNADP-IDH |
Description: Anti IDH2(R172K) Mouse Monoclonal Antibody |
Background: Isocitrate dehydrogenase (IDH) catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. The isocitrate and isopropylmalate dehydrogenases family has three members, IDH1, IDH2 and IDH3. IDH2 plays a role in intermediary metabolism and energy production. Defects in IDH2 are the cause of D-2-hydroxyglutaric aciduria type 2 (D2HGA2). Somatic mosaic mutations of this protein have also been found associated to Ollier disease and Maffucci syndrome, and R172K IDH2 mutations do exist in diffusely infiltrative gliomas. |
Immunogen: A synthetic peptide from the internal region of IDH2 which includes the mutation of R172K, human origin. |
Applications: ELISA, WB, IHC |
Recommended Dilutions: ELISA: 1:1000-1:5000 WB: 1:100-1:1000 IHC: 1:50-1:100 |
Concentration: 1 mg/ml |
Host: Mouse |
Format: Liquid |
Clonality: Monoclonal |
Isotype: |
Purity: Purified from ascites |
Storage buffer: |
Preservative: no |
Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
Species Reactivity: recognizes R172K mutant, but not wild-type IDH2 of vertebrates. |
Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles |
Western blot: |
Western blot analysis of recombinant IDH2(R172K) and wildtype proteins. Purified His-tagged IDH2(R172K) (lane 2) and wildtype protein (lane 1) were blotted with Anti IDH2(R172K) monoclonal antibody (Cat. #26163). |
Immunofluorescence: |
Immunofluorescence of cells expressing IDH2 proteins with Anti IDH2(R172K) antibody. HEK293T cells were transfected with pCDNA3-GFP- IDH2(R172K) plasmid , pCDNA3-GFP IDH2(WT) plasmid, pCDNA3-GFP IDH2(R172H) plasmid , pCDNA3-GFP IDH2(R172W) plasmid, pCDNA3-GFP IDH2(R172G) plasmid,pCDNA3-GFP IDH2(R172M) plasmid or pCDNA3-GFP IDH2(R172S) plasmid, then fixed and stained with anti- IDH2(R172K) monoclonal antibody (Cat. #26163) |
Related Products | |||
Catalog# | Name | Size | Price |
26231 | Anti IDH2(R172G) Mouse Monoclonal Antibody | 100 μL | ¥4800 |
26164 | Anti IDH2(R172W) Mouse Monoclonal Antibody | 100 μL | ¥4,800 |
26163 | Anti IDH2(R172K) Mouse Monoclonal Antibody | 100 μL | ¥4,800 |
26408 | Anti IDH2(R172S) Mouse Monoclonal Antibody | 100 μL | ¥4800 |
26165 | Anti IDH2(R140Q) Mouse Monoclonal Antibody | 100 μL | ¥4,800 |
26231 | Anti IDH2(R172G) Mouse Monoclonal Antibody | 100 μL | ¥4,800 |
26084 | Anti IDH2(R172H) Mouse Monoclonal Antibody | 100 μL | ¥4800 |
發(fā)表時(shí)間:2015
名稱/貨號(hào):Anti- IDH2(R172K) Mouse Monoclonal Antibody /26163
影響因子:J Neurochem IF=4.5
應(yīng)用范圍: WB IHC
種屬:Human
發(fā)表時(shí)間:2016
名稱/貨號(hào):Anti- IDH2(R172K) Mouse Monoclonal Antibody /26163
影響因子:PLoS One IF=2.9
應(yīng)用范圍:IHC(作為臨床診斷的應(yīng)用工具)
種屬:Human (神經(jīng)膠質(zhì)瘤)
發(fā)表時(shí)間:2019-07-11
名稱/貨號(hào):Anti- IDH2(R172K) Mouse Monoclonal Antibody /26163
影響因子:Mod Pathol IF=7.1
應(yīng)用范圍:IHC(作為臨床診斷的應(yīng)用工具)
種屬:Human (T細(xì)胞淋巴癌)
發(fā)表時(shí)間:2020-12
名稱/貨號(hào):Anti- IDH2(R172K) Mouse Monoclonal Antibody/26163
影響因子:Hum Pathol IF=2.7
應(yīng)用范圍:IHC(作為臨床診斷的應(yīng)用工具)
種屬:Human(神經(jīng)膠質(zhì)瘤)
發(fā)表時(shí)間:2021-01-11
名稱/貨號(hào):Anti- IDH2(R172K) Mouse Monoclonal Antibody /26163
影響因子:The American Journal of Surgical Pathology IF=5.6
應(yīng)用范圍:IHC(作為臨床診斷的應(yīng)用工具)
種屬:Human (淋巴癌)
技術(shù)支持:優(yōu)狐