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Anti- NFE2L2(L30F) Mouse Monoclonal Antibody

價(jià)格¥4800.00
品牌NewEast Biosciences   
產(chǎn)地中國(guó) 武漢
貨號(hào)26332
規(guī)格100μl
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  • NFE2L2(L30F) [26332]
    [100ul]

     

    Catalog Number:  26332
    Gene Symbol:  NFE2L2, NRF2
    Description:   Anti NFE2L2(L30F) Mouse Monoclonal Antibody
    Background:   NFE2L2 protein is encoded by NFE2L2 gene. It is a basic leucine zipper protein that regulates the expression of antioxidant proteins that protect against oxidative damage triggered by injury and inflammation. Several drugs that stimulate the NFE2L2 pathway are being studied for treatment of diseases that are caused by oxidative stress.
    Immunogen:   A synthetic peptide from the internal region of NFE2L2 which includes the mutation of L30F, human origin.
    Applications:   ELISA, WB, IHC
    Recommended Dilutions:   
    ELISA: 1:1000-1:2000 
    WB: 1:500-1:1000 
    IHC: 1:50-1:100
    Concentration:   1 mg/ml
    Host:   Mouse
    Format:   Liquid
    Clonality:   Monoclonal
    Isotype:   
    Purity:   Purified from ascites
    Storage buffer:
    Preservative:  no
    Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
    Species Reactivity:  Recognizes L30F mutant, but not wild-type NFE2L2 of vertebrates.
    Storage Conditions:   Store at -20°C. Avoid freeze / thaw cycles
     
    Western blot:  
    Western blot analysis of recombinant NFE2L2(L30F) and wildtype proteins. Purified His-tagged NFE2L2(L30F) protein (lane2) and corresponding wild-type protein (lane1) were blotted with Anti NFE2L2(L30F) monoclonal antibody (Cat. #26332).
    Immunofluorescence:  
    Immunofluorescence of cells expressing NFE2L2 proteins with Anti NFE2L2(L30F) antibody. HEK293T cells were transfected with pCDNA3-GFP NFE2L2(WT) plasmid (left column) or pCDNA3-GFP NFE2L2(L30F) plasmid (right column), then fixed and stained with Anti NFE2L2(L30F) monoclonal antibody (Cat. #26332).

     

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