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Anti- BRCA1(C61G) Mouse Monoclonal Antibody

價(jià)格¥4800.00
品牌NewEastBio   
產(chǎn)地中國(guó) 武漢
貨號(hào)26319
規(guī)格100ul
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  • BRCA1(C61G) [26319]
    [100ul]

     

    Catalog Number:  26319
    Gene Symbol:  BRCA1, BRCC1, IRIS, PNCA4, PPP1R53, PSCP
    Description:   Anti BRCA1(C61G) Mouse Monoclonal Antibody
    Background:   BRCA1 is normally expressed in cells of breast and other tissues, where it helps repair damaged DNA or destroy cells if DNA cannot be repaired. It is involved in the repair of chromosomal damage with an important role in the error-free repair of DNA double-strand breaks. If BRCA1 itself is damaged by a BRCA1 mutation, damaged DNA is not repaired properly, and this increases the risk for breast cancer.
    Immunogen:   A synthetic peptide from the internal region of BRCA1 which includes the mutation of C61G, human origin.
    Applications:   ELISA, WB, IHC
    Recommended Dilutions:   
    ELISA: 1:1000-1:2000 
    WB: 1:500-1:1000 
    IHC: 1:50-1:100
    Concentration:   1 mg/ml
    Host:   Mouse
    Format:   Liquid
    Clonality:   Monoclonal
    Isotype:   
    Purity:   Purified from ascites
    Storage buffer:
    Preservative:  no
    Constituents:  PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
    Species Reactivity:  Recognizes C61G mutant, but not wild-type BRCA1 of vertebrates.
    Storage Conditions:   Store at -20°C. Avoid freeze / thaw cycles
     
    Western blot:  
    Western blot analysis of recombinant BRCA1(C61G) and wildtype proteins. Purified His-tagged BRCA1(C61G) protein (lane2) and corresponding wild-type protein (lane1) were blotted with Anti BRCA1(C61G) monoclonal antibody (Cat. #26319).
    Immunofluorescence:  
    Immunofluorescence of cells expressing BRCA1 proteins with Anti BRCA1(C61G) antibody. HEK293T cells were transfected with pCDNA3-GFP BRCA1(WT) plasmid (left column) or pCDNA3-GFP BRCA1(C61G) plasmid (right column), then fixed and stained with Anti BRCA1(C61G) monoclonal antibody (Cat. #26319).

     

    Related Products
    Catalog# Name Size Price
    26319 Anti BRCA1(C61G) Mouse Monoclonal Antibody  100 μL  ¥4800 
    26351-1 Anti BRCA1(1-120) Mouse Monoclonal Antibody  100 μL  ¥4800 
    26351-2 Anti BRCA1(1720-1863) Mouse Monoclonal Antibody  100 μL  ¥4800 

     

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