neweastbio@126.com
PRODUCT CENTER
價(jià)格 | ¥4800.00 |
品牌 | NewEast Biosciences |
產(chǎn)地 | 中國(guó) 武漢 |
貨號(hào) | 21202 |
規(guī)格 | 100ul |
說明書 | 說明書下載 |
ERBB2 [21202]
[100 µL]
Catalog Number: 21202 |
Gene Symbol: ERBB2/HER2/Neu |
Description: Anti ErbB2 Rabbit Polyclonal Antibody |
Background: The ErbB family is composed of four plasma membrane-bound receptor tyrosine kinases. Amplification or over-expression of the ERBB2 gene occurs in approximately 30% of breast cancers. HER2 is encoded by ERBB2, a known proto-oncogene located at the long arm of human chromosome 17(17q21-q22). HER2 proteins have been shown to form clusters in cell membranes that may play a role in tumorigenesis. |
Immunogen: A synthetic peptide from the central of ErbB2 protein. |
Applications: ELISA, WB, IHC |
Recommended Dilutions: ELISA: 1:500-1:5000 WB: 1:200-1:2000 IHC: 1:20-1:200 |
Concentration: 0.2 mg/ml |
Host: Rabbit |
Format: Liquid |
Clonality: Polyclonal |
Isotype: |
Purity: Purified from serum |
Storage buffer: |
Preservative: no |
Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
Species Reactivity: Recognizes ErbB2 of vertebrates. |
Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles. |
Western blot: |
Western blot analysis of ErbB2 protein. Purified full length His-tagged ErbB2 protein (Cat. #10208) was blotted with Anti ErbB2 rabbit polyclonal antibody (Cat. #21202). |
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Anti ErbB2 polyclonal antibody (Cat. #21202). Tissue samples were fixed with formaldehyde and blocked with 1% serum for 15 min at 37 °C. Antigen retrieval was by heat mediation in citrate buffer (pH6). Samples were then incubated with primary antibody (1:50 dilution) overnight at 4 °C. A HRP-conjugated Goat anti-Rabbit IgG (1:50) was used as secondary antibody. |
Related Products | |||
Catalog# | Name | Size | Price |
21200 | Anti ERBB2 Rabbit polyclonal Antibody | 100 μL | ¥2600 |
21202 | Anti ERBB2 Rabbit Polyclonal Antibody | 100 μL | ¥2600 |
26080 | Anti ERBB2(V777A) mouse monoclonal Antibody | 100 μL | ¥4800 |
26127 | Anti ERBB2 mouse monoclonal Antibody | 100 μL | ¥2800 |
技術(shù)支持:優(yōu)狐