neweastbio@126.com
PRODUCT CENTER
價(jià)格 | ¥4800.00 |
品牌 | NewEast Biosciences |
產(chǎn)地 | 中國(guó) 武漢 |
貨號(hào) | 26082 |
規(guī)格 | 100ul |
說(shuō)明書(shū) | 說(shuō)明書(shū)下載 |
EGFR(L858R) [26082]
[100 µL]
Catalog Number: 26082 |
Gene Symbol: EGFR/HER1/ERBB1 |
Description: Anti EGFR(L858R) Mouse Monoclonal Antibody |
Background: EGFR is a receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. It activates at least 4 major downstream signaling cascades including the RAS RAF MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. Defects in EGFR are associated with lung cancer (LNCR). |
Immunogen: A synthetic peptide from the internal region of EGFR which includes the mutation of L858R, human origin. |
Applications: ELISA,WB,IF,IHC |
Recommended Dilutions: ELISA: 1:1000-1:5000 WB: 1:1000-1:5000 IHC: 1:50-1:100 |
Concentration: 1 mg/ml |
Host: Mouse |
Format: Liquid |
Clonality: Monoclonal |
Isotype: IgG1 |
Purity: Purified from ascites |
Storage buffer: |
Preservative: no |
Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol |
Species Reactivity: Recognizes EGFR(L858R), but not wild type EGFR from vertebrates. |
Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles. |
Western blot: |
Western blot analysis of recombinant EGFR(L858R) and wildtype proteins. Purified His-tagged EGFR(L858R) protein (amino acid 702-987, lane 2, Cat. # 10230) and corresponding wild type protein (amino acid 702-987, lane 1, Cat. # 10210) were blotted with anti EGFR(L858R) mouse monoclonal antibody (Cat. # 26082) . Proteins loading (bottom) were blotted with anti-His-tag monoclonal antibody (Cat. # 28004). |
Immunofluorescence: |
Immunofluorescence of cells expressing EGFR proteins with anti EGFR(L858R) antibody. HEK293T cells were transfected with pCDNA3-GFP EGFR(WT) plasmid (left column) or pCDNA3-GFP EGFR(L858R) plasmid (right column), then fixed and stained with anti- EGFR(L858R) monoclonal antibody (Cat. # 26082). |
Immunohistochemistry: |
Immunohistochemical analysis of paraffin-embedded human NSCLC tissue with anti-EGFR(L858R) monoclonal antibody (Cat. #26082). Tissue samples were fixed with formaldehyde and blocked with 1% serum for 15 min at 37 °C. Antigen retrieval was by heat mediation in citrate buffer (pH6). Samples were then incubated with primary antibody (1:400) overnight at 4°C. A HRP-conjugated Goat anti-mouse IgG (dilution 1:100) was used as secondary antibody. |
Related Products | |||
Catalog# | Name | Size | Price |
26287 | Anti EGFR(L747-P753)S Mouse Monoclonal Antibody | 100 μL | ¥4800 |
26119 | Anti EGFR(G863D) Mouse Monoclonal Antibody | 100 μL | ¥4800 |
26083 | Anti EGFR(G719S) Mouse Monoclonal Antibody | 100 μL | ¥4800 |
26082 | Anti EGFR(L858R) Mouse Monoclonal Antibody | 100 μL | ¥4800 |
26077 | Anti EGFR(L861Q) Mouse Monoclonal Antibody | 100 μL | ¥4800 |
26115 | Anti EGFR(G719A) Mouse Monoclonal Antibody | 100 μL | ¥4800 |
26120 | Anti EGFR(D761Y) Mouse Monoclonal Antibody | 100 μL | ¥4800 |
26121 | Anti EGFR(V769L) Mouse Monoclonal Antibody | 100 μL | ¥4800 |
26117 | Anti EGFR(A839T) Mouse Monoclonal Antibody | 100 μL | ¥4800 |
26116 | Anti EGFR(N826S) Mouse Monoclonal Antibody | 100 μL | ¥4800 |
26176 | Anti EGFR(vIII) Mouse Monoclonal Antibody | 100 μL | ¥4800 |
26221 | Anti EGFR(L858Q) Mouse Monoclonal Antibody | 100 μL | ¥4800 |
26481 | Anti EGFR(T854A) Mouse Monoclonal Antibody | 100 μL | ¥4800 |
26483 | Anti EGFR(S768I) Mouse Monoclonal Antibody | 100 μL | ¥4800 |
發(fā)表時(shí)間:2015
名稱/貨號(hào):Anti- EGFR(L858R) Mouse Monoclonal Antibody/ 26082
影響因子:Tumour Biol
應(yīng)用范圍:IHC(作為臨床診斷的應(yīng)用工具)
種屬:Human(肺腺癌)
技術(shù)支持:優(yōu)狐